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Synthetic gene using the assembly of synthetic oligonucleotides often comprises two steps: step 1, divide the long gene to several fragments;step2,assemble the overlapping oligonucleotides into a long gene fragment by polymerase chain assembly(PCA) in a PCR.BitOligo mainly contains two functions:1) gene analysis for dividing long sequence into short fragments;Oligo design to generating oligo library for PCA.

Features of BitOligo

Platforms:Window XP/Vista/window 7

User guide
PCR reaction parameters: User need change the following parameters based on the PCR condition:Overlap Tm and concentrations of variable of components such as dNTPs.

Algorithm parameters:

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