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Run Codon Score

Gene expressing is modulated frequently in translation procedure.Ribosomes prefer to choose the minority of codons in life evolution.Mining the pattern of gene expression in target host is a very challenging work to bioinformaticans.Fortuntely, BitGene senior scientist, Firebird, developped the algorithm to evaluate gene expression level: Codon_score. Codon_score employs a scoring model,which is based on gene synthetic database:SGDB[3]. The below two cases show that Codon_score can predict the gene expression level accurate.

References:

[1] Brian Seed,1998,Overexpression of mammalian and viral proteins,US patent.Patent Number:5786464

[2] Deml.et al,2001,Multiple Effects of Codon Usage Optimization on Expression and Immunogenicity of DNA Candidate Vaccines Encoding the Human Immunodeficiency Virus Type 1 Gag Protein,J Virol.75(22):10991-1001

[3]Wu .et al,2007,SGDB: a database of synthetic genes re-designed for optimizing protein over-expression,Nucleic Acids Res.v35

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